GO Transit buses operate Monday through Saturday. Listed below are the service hours by route. There is no bus service on the following holidays: New Year’s Day, Memorial Day, July 4th, Labor Day, Thanksgiving, Christmas, or days celebrated as such.
| Route | Service Hours | Beginning and Ending Point |
| 1 | 6:15 AM - 6:45 PM | Mallard & Grove |
| 2 | 6:15 AM - 6:45 PM | Smith/North High |
| 3 | 6:15 AM - 6:15 PM | New York & Elmwood |
| 4 | 6:30 AM - 6:30 PM | Logan & Jackson |
| 5 | 6:15 AM - 6:45 PM | West Transfer Point |
| 6 | 6:15 AM - 6:45 PM | Walmart |
| 7 | 6:15 AM - 6:15 PM | West Transfer Point |
| 8 | 6:15 AM - 6:45 PM | Target |
| 9 | 6:15 AM - 6:45 PM | Walmart |
Route 10 service to Neenah operates Monday - Friday from 6:30 AM - 6:30 PM. On Saturday, route 10 operates from 7:45 AM - 6:30 PM.